What we used:
Feather- Used to give the Leech skin a very gentle touch stimulation
Probe- Used for lifting, pushing, pressing
Forceps- Fine forceps for very fine manipulation
Scissors- Used to cut the Leech
Pins- Pinning tissue to the dissecting dish
Scalpel- Used to cut many thing
Dissection tray- To stick pins into it for any stabilize specimen for dissection
Leech tank- Keep leech in pond water
20% ethanol- Used to anesthetize the leech
Leech tongs- Used to pick up the leech
Dissection microscope- Binocular microscopes specifically for dissection and other micromanipulations
Micromanipulator- Used to position items with sub-micrometer precision in three dimensions
Oscilloscope- Its like a sophisticated volmeter
Leech- Test subject
Leech- Test subject
Here is what I did...
I first grab the leech with the leech tongs and dip it in 20% ethanol solution to anesthetize it. I then stretched out the leech and pin it to the dissection tray. After I pinn the leech I then cut it open with the scissors. Using the forceps to carefully tease apart the skin. With the probe removed the guts and other stuff to expose the ventrally located nerve cord. When everything has been removed then go to the dissection microscope, here you'll be using the scalpel to make incision. Then cut a little piece of the leech and pin it to the dissection tray I headed to the dissection microscope. After that the micromanipulator came to action. Everything before this action was only for preparation for it. I first used a feather, then the probe and lastly the forceps to test the cell response. After I click on Dye Injection and then the UV Switch to identify the cell I got.
Down Below is the Cell Type that I could have gotten.
On my first attempt I got R and on my last attempt I got T.
In this lab I learn a lot about the leech and the nervous system.
Click here if you'll like to do your own little attempt and see where I got all my information for this lab.
